Journal: International Immunology

Article Title: Integrin CD11b provides a new marker of pre-germinal center IgA + B cells in murine Peyer’s patches

doi: 10.1093/intimm/dxab113

Figure Lengend Snippet: CD11b + IgA + B cells are pre-GC B cells interacting with CD4 + T cells. (A) Heatmap indicating the log fold change (CD11b + IgA + B cells/CD11b − IgA + B cells) in expression of indicated genes by microarray analysis. (B) Expression levels of indicated GC specific genes and non-GC specific genes and a pre-GC gene were analyzed by qPCR and normalized to those from the β-actin gene. The mean of relative expression levels of CD11b − IgA + B cells is taken as 1. Each spot represents the mean of technical triplicates. Bar graphs show the mean values (±SD) of three or four independent measurements. Data were analyzed by ANOVA followed by Tukey’s multiple comparisons. (C) Flow cytometry histograms depicting expression of surface markers of CXCR4 and CD86 in CD11b + IgA + B cells and CD11b − IgA + B cells. Representative data are from at least five independent experiments. (D) Flow cytometry data of singlet IgA + B cells and conjugated CD4 + IgA + cells. (E) Flow cytometry data of CD11b − IgA + and CD11b + IgA + PP B cells with CD4 + T cells in the conjugated gate. (F) Percentage of the CD4 + T cells in conjugation with CD11b + IgA + and CD11b − IgA + PP B cells. Bar graphs show the mean values (±SD) of six independent measurements. Data were compared by two-tailed unpaired Student’s t test. (G) Imaging of sorted CD11b + IgA + PP B cells conjugating with CD4 + T cells. Scale bar, 20 μm.

Article Snippet: Microarray analysis was performed with a SurePrint G3 Mouse GE v2 8x60K Microarray Chip (Affymetrix) to detect the gene expression.

Techniques: Expressing, Microarray, Flow Cytometry, Conjugation Assay, Two Tailed Test, Imaging